HPLC - UV/VIS Spectroscopy
Fiber Optic and Diode Array Applications
Software - Fluorescence
| Overview | Specifications | Ordering Information | Print Version | Documentation |
This highly innovative automatic Amino Acid Analyzer combines the advantages of the classical ion exchange separation method with the modern techniques of high performance liquid chromatography. The complete package of sophisticated instrumentation, a wide variety of prepacked and tested separation columns, combined with optimized ready-to-use buffer solutions and chemicals, creates the right answer for any routine or research problem in amino acid determination.More than 30 years experience in developing and operating sophisticated amino acid analyzers results in unmatched performance.
Multistep Separations
Only two or three buffer solutions need to be combined to form an optimized buffer profile in any section of the separation program resulting in a very large improvement in flexibility for difficult separations.
Cooled Reagent Storage
All buffer solutions, as well as the Ninhydrine reagent are stored under inert gas pressure in a refrigerated cabinet to avoid oxidation and air contamination independent of the ambient environment.
Integrated Autosampler
Volumes from 1µl to 100 µl can be injected without any loss of sample, a capacity for 120 vials in a cooled sample tray is available. A programmable wash program will flush the entire injection system to avoid cross contamination.
Programmable Column Oven
A programmable solid state column oven with fast heating and cooling capability with a temperature range from +5°C to +85°C independent of the ambient temperature is fitted as standard on the analyzer.
High-Temperature Reactor
A programmable reaction module with a temperature range from ambient to 185°C with a coiled capillary for the color reaction of the amino acid-ninhydrine complex. Automatic flushing of the reactor coil with a washing solution after each run prevents the blockage of the capillary.
Integrated Reagent Dosing Pump
A special reagent pump is used to deliver Ninhydrine and flush the reaction coil after each run. Flowrates from 0.01 to 2.0 ml/min. are programmable.
Completely Inert Design
All materials coming into contact with the buffer solutions and reagents are made of inert materials such as PEEK, PTFE, PVDF etc., therefore, there is no need to consider using special non-corrosive buffer recipies or reagents.
Intuitive Software Control
Controlling the complex features of the S433 is made easy using intuitive software with all status parameters displayed on one screen. Additional details such as gradient program steps or column oven temperatures can be displayed as separated tables. The use of an intuitive screen layout helps new users to start working with this software faster than ever before plus offering the following features:.
- Complete control of Gradient Program, Column and Reactor Temperature
- Programmable Sample Sequence with individual run times, gradient and temperature profiles
- Automatic usage control of Buffer and Reagent solutions
- Emergency-Program (SOS) feature in case of pressure errors
- Manual control of each single unit
| Solvent Delivery System S 2100 | ||||
| Pump type | Dual plunger pump with special design for low pulsation at less than 1 %) | |||
| Gradient system | Quaternary low pressure system with integrated 4-channel vacuum degasser | |||
| Flow range | Depending on pump head installed, typically 0.05 to 9.99 ml/min | |||
| Pressure | Up to maximum 350 bar (5200 PSI) | |||
| Program storage | Battery buffered, graphic display of gradient profile A, B, C, and D with programmable mixing cycle for the buffer solution | |||
| Pressure control | High and low pressure control limits | |||
| Autosampler | ||||
| Injection mode | Fixed and variable volume up to 100 µl | |||
| Mode of operation | X Y Z transfer module | |||
| Number of samples | Up to 120 | |||
| Pre-column derivatisation | Optional | |||
| Dosing syringe | Exchangeable | |||
| Sample cooling | Integrated Peltier based module | |||
| Column Oven | ||||
| Design | Solid state with fast heating and cooling capability | |||
| Temperature range | +5°C to +85°C | |||
| Programming | Up to twelve temperature steps can be used during one separation program | |||
| Reaction and Detection Module | ||||
| Temperature range | Programmable from ambient to 185°C | |||
| Reagent Dosing Pump | Programmable flow rate from 0.01 to 2.0 ml/min. | |||
| Flushing | Automatic flushing of the reactor coil with a washing solution after each run to prevent blockage of the capillary | |||
| Detection module | Integrated dual-channel photometer for the detection of amino acids at 440 and 570 nm | |||
| Reagent Organizer | ||||
| Protection | Inert gas (N2) supply with adjustable pressure to prevent buffer/reagent oxidation and contamination | |||
| Temperature control | Integrated cooling module | |||
| Analytical Specifications | ||||
| Hydrolysate | Hydrolysate sample: under 60 min. runtime | |||
| Physiological | Physiological sample: under 120 min. runtime | |||
| Sensitivity | 10 pMol | |||
| Optional Applications | ||||
| OPA | Post column derivatisation with OPA (needs an optional Fluorescence Detector) | |||
| Carbohydrates | Carbohydrate determination for reducing sugars with Cu-bicinchoninate post-column derivatisation (except the separation column, no additional extras are needed) | |||
| Polyamines | Polyamine determination with Ninhydrine or OPA post-column derivatisation | |||
| All specifications may be subject to change for technical reasons. | ||||
| 11 20 001 | Automatic Amino Acid Analyzer S 433 without data system |
| 11 20 002 | Automatic Amino Acid Analyzer S 433-D with data system |
| 11 20 003 | Manual Amino Acid Analyzer S 430 without data system |
| 11 20 004 | Manual Amino Acid Analyzer S 430-D with data system |