HPLC - UV/VIS Spectroscopy  Fiber Optic and Diode Array Applications  Software - Fluorescence
> ION ANALYSIS
Overview Specifications Ordering Information Print Version Documentation
Overview
For the separation of ionic components both chromatographic and electrophoretic techniques are used. Isotachophoresis (ITP) exploits differences in electrophoretic mobility in the presence of an electric field to effect the separation of ionic substances.   Non-continuous Electrolyte System Characteristic for ITP is the use of a non-continuous system, which consists of two aqueous electrolytes, the so-called leading and trailing electrolytes. This system is usually realised within capillaries with internal diameters from 200 - 300 µm, in order to minimise heating due to the electric current and allow very high separation efficiencies to be achieved.   Minimum Sample Preparation With chromatographic techniques the flow of the mobile phase usually causes the sample matrix to also be transported through the analytical column. This has the disadvantage, that often expensive and time-consuming sample preparation steps are required, before the analysis can be started.   In isotachophoresis it is generally possible to define conditions such that the sample matrix is not transported through the analytical separation path. The advantage of this is that practically all samples can be injected either directly or require only a simple dilution step before analysis can be started.   Application Possibilities Isotachophoresis can in most cases be considered and used as a viable alternative to conventional ion chromatography. ITP brings maximum advantage when used for the analysis of complex matrixes. Typical examples in these areas are:   Organic acids in silage and fermentation Trace impurities in H2O2, Glycerine, Inks Anions in urine and serum Taste enhancers, acidifiers, vitamins, food additives Inorganic anions and cations in ground, surface or drinking water Active agents and metabolites in creams and tablets Proteins and amino acids Additional information on Isotachophoresis is to be found under the Documentation link accessible from this page.
Specifications
Separation zone Two coupled capillaries Pre-separation capillary 800 µm I.D., FEP Separating capillary 300 µm I.D., fused silica Detection Up to three detectors possible depending on version supplied Detection stage 1 Contact-free conductivity detector integrated between the two capillaries Detection stage 2 Contact-free conductivity detector located after the second capillary UV Detector Measuring cell integrated with the separating capillary Deuterium lamp as light source 4 wavelengths: 200, 220, 254, 280 nm Fiber-optic coupling Dimensions Manual unit: 400 x 685 x 400 mm (W x H x D) Automatic unit: 705 x 685 x 460 mm (W x H x D) Weight Manual unit: 20 kg Automatic unit: 35 kg Options Diode array detector Available as an option: Range 190 - 1100 nm, 1024 pixel, 0.8 nm/pixel, using fiber-optic coupling Autosampler The Basic MARATHON and the TRIATHLON autosamplers are supported All specifications may be subject to change for technical reasons.
Ordering Information
81 657 02 ItaChrom II – Manual Capillary Electrophoresis System Separation unit: coupling of 2 capillaries Detection: Two conductivity detectors and one UV detector with light guides Control: PC controlled including software for Win95/98/NT 81 657 01 ItaChrom II – Automated Capillary Electrophoresis System Separation unit: coupling of 2 capillaries Detection: Two conductivity detectors and one UV detector with light guides Electrolyte unit: automated filling system for electrolyte Autosampler: MARATHON (96 Vials) Control: PC controlled including Software requires minimum WinNT4 SP5) Spare Parts 51 595 43 Pre-capillary, complete 90 mm/0,8 51 595 42 Exchange detector, pre-column 51 595 45 Separating capillary, complete 160 mm/0,3 52 001 00 Exchange detector, separating column 51 595 57 ITP Injection valve 52 001 01 Exchange valve insert 51 595 53 Bifurcation block 52 001 02 Reservoir 52 001 03 Filling tube with screw 51 553 72 Cellophane membrane 52 001 04 Fixing ring 52 001 05 Screw 51 595 59 O-Ring 52 001 06 Needle valve 52 001 07 Flange 52 001 08 Washer 52 001 09 Waste container 52 001 10 Injection valve holder 52 001 11 Valve body 52 001 12 TE Reservoir 52 001 13 High voltage unit 52 001 14 HV Relay 52 001 15 Cable set 52 001 16 Oscillator card 51 595 51 Controller 51 595 52 Detector card 52 001 17 LV Power supply 52 001 18 Replacement lamp (254 nm) 51 595 40 Replacement lamp (280 nm) 52 001 19 UV Sensor 51 595 49 UV Filter including holder and lense 51 595 50 Lamp holder 52 001 20 ITP Valve 300 µl 52 001 21 CZE Valve 51 595 61 Microprep. valve ITP Electrolytes 51 595 01 Leading electrolyte A1 pH = 6 / 250 ml 51 595 02 Leading electrolyte A2 pH = 3.2 / 250 ml 51 595 03 Leading electrolyte A3 pH = 3.5 / 250 ml 51 595 04 Leading electrolyte A4 pH = 3.8 / 250 ml 51 595 05 Leading electrolyte A5 pH = 4.5 / 250 ml 51 595 06 Leading electrolyte A6 pH = 6 / 250 ml 51 595 07 Leading electrolyte A7 pH = 9 / 250 ml 51 595 17 Leading electrolyte A8 pH = 3.6 / 250 ml 51 595 10 Trailing electrolyte A1 pH = 6 / 100 ml 51 595 25 Trailing electrolyte A1 pH = 6 / 250 ml for automated ITP 51 595 11 Trailing electrolyte A2 pH = 6 / 100 ml 51 595 26 Trailing electrolyte A2 pH = 6 / 250 ml for automated ITP 51 595 12 Trailing electrolyte A3 pH = 9 / 100 ml 51 595 19 Trailing electrolyte A4 pH = 5 / 100 ml 51 595 27 Trailing electrolyte A4 pH = 5 / 250 ml for automated ITP 51 595 20 Trailing electrolyte A5 pH = 4 / 100 ml 51 595 08 Leading electrolyte C1 pH = 5.2 / 250 ml 51 595 09 Leading electrolyte C2 pH = 5.4 / 250 ml 51 595 18 Leading electrolyte C3 pH = 5.2 / 250 ml 51 595 13 Trailing electrolyte C1 pH = 3 / 100 ml 51 595 14 Trailing electrolyte C2 pH = 5 / 100 ml 51 595 21 Trailing electrolyte C3 pH = 5 / 100 ml 51 595 15 Background electrolyte CZE pH = 5.2 / 250 ml 51 595 16 Anti-convection reagent MHEC / 100 ml 51 595 22 Leading electrolyte A9 pH = 3.5 / 250 ml 51 595 23 Trailing electrolyte A10 pH = 6 / 250 ml

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