HPLC - UV/VIS Spectroscopy
Fibre Optic and Diode Array Applications
Software - Fluorescence
| Overview | Specifications | Ordering Information | Print Version | Documentation |
LifePower TM-Light is a software program designed to acquire and store data for the evaluation of the meltpoint of a nucleic acid sample. Data generated with this software package can be saved, printed or exported in ASCII or EXCEL format to be used for further calculations.Melting Temperature (Tm) of Nucleic Acids
The melting temperature (Tm) value and the shape of the thermal transition curve depends on the sequence and length of the nucleic acids for given experimental conditions (ionic strength, pH, etc).
Automated Acquisition
Once the thermal experiment starts, LifePower TM-light continuously acquires the absorbance value at the corresponding temperature at regular user-selected time or temperature intervals until the final temperature has been reached.
Online Display
Curve graphics are displayed in real time automatically and updated during data acquisition, at the end of the experiment, the displayed raw data can be processed and normalized.
Reportable Results
Absorbance vs. temperature data with autoscaled graphs and real-time data storage are provided. User defined comments can be stored within the data file and all entered parameters are saved as a customized command file or as default parameters for the next experiment.
Complete Data
The DNA thermal melting curve will provide not only the melting temperature (Tm), but also the free energy and its associated enthalpy and entropy contributions.
| Common Features | Online control of spectralphotometer and thermosystem | |||
| Definition of temperature gradients | ||||
| Extraction to ASCII or Excel with post-processing capability | ||||
| Database | All experimental data can be stored in a database supplied with the software | |||
| Compressed data storage | ||||
| Comfortable search functions | ||||
| 2D - Methods | Real time data acquisition of absorbance versus temperature and wavelength | |||
| Max. 6 wavelengths and 10 samples in parallel | ||||
| Automatic basepoint correction | ||||
| Additional scans during an experiment | ||||
| Numerical or graphical display with multiview | ||||
| 3D - Methods | Acquisition of scans (wavelength versus absorbance) versus temperature | |||
| Max. 10 samples in parallel | ||||
| Graphical 3D display with either line or surface view | ||||
| 3D - rotation, zoom, panning | ||||
| Extraction of single scan for a defined temperature | ||||
| Extraction of single temperatures for a defined wavelength | ||||
| Hardware Requirements | ||||
| Processor | Pentium III or better | |||
| Memory | 256 MB RAM, min. 10 GB Harddisk | |||
| Operating system | Windows NT 4.0 SP6, Windows 2000 SP4 or Windows XP | |||
| All specifications may be subject to change for technical reasons. | ||||
| 91-91694 | LifePower TM DNA Melting Curve Software for UVIKON XS and XL |