Overview
LifePower TM-Light is a software program designed to acquire and store data for the evaluation of the meltpoint of a nucleic acid sample. Data generated with this software package can be saved, printed or exported in ASCII or EXCEL format to be used for further calculations.
Melting Temperature (Tm) of Nucleic Acids
The melting temperature (Tm) value and the shape of the thermal transition curve depends on the sequence and length of the nucleic acids for given experimental conditions (ionic strength, pH, etc).
Automated Acquisition
Once the thermal experiment starts, LifePower TM-light continuously acquires the absorbance value at the corresponding temperature at regular user-selected time or temperature intervals until the final temperature has been reached.
Online Display
Curve graphics are displayed in real time automatically and updated during data acquisition, at the end of the experiment, the displayed raw data can be processed and normalized.
Reportable Results
Absorbance vs. temperature data with autoscaled graphs and real-time data storage are provided. User defined comments can be stored within the data file and all entered parameters are saved as a customized command file or as default parameters for the next experiment.
Complete Data
The DNA thermal melting curve will provide not only the melting temperature (Tm), but also the free energy and its associated enthalpy and entropy contributions.
Specifications
